![]() ![]() It relies on reading two standards for calibration every time that you make measurements. The Qubit spectrophotometer uses a DNA dye whose fluorescence changes upon binding double-stranded DNA. Not Recommended for RSF1010-based plasmids or other samples containing significant amounts of single-stranded DNA (e.g., oligonucleotides). Recommended Method for gDNA preps, purified PCR products, NGS library prep, and low-concentration plasmid preps. Most time consuming Low dynamic range and sensitivity May significantly overestimate concentrations if a sample has multiple DNA species or high levels of other chemicals, including salts Requires 2 point standard curve to measure even one sample Only accurate for dsDNA with typical kits used Robust to contaminants Can detect mixed DNA species and determine the concentrations of multiple DNA species in a mixture Quickest Largest dynamic range Works for both ssDNA and dsDNA samples General UV Spectrophotometer Nucleic Acid Quantitation Protocol.NanoDrop (or other UV Spectrophotometer).Biotium AccuGreen DNA BR (Broad Range) Protocol. ![]()
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